Lovinger G G, Klein R, Ling H P, Gilden R V, Hatanaka M
J Virol. 1975 Oct;16(4):824-31. doi: 10.1128/JVI.16.4.824-831.1975.
Replicating transforming functions of Rauscher leukemia virus (RLV) and the RLV pseudotype of Moloney sarcoma virus in mouse embryo fibroblasts were found to be most sensitive to inhibition by cytosine arabinoside (ara-C) 30 to 90 min after infection. The initiation of intracellular RLV DNA synthesis was detected by nucleic acid hybridization within this time interval. Treatment of infected cells with cytosine arabinoside abolished RLV DNA synthesis. Peak synthesis of the DNA complementary to the infecting RLV genome, the (-) strand, occurred 40 to 60 min after infection. During this interval two s two species of DNA were observed with estimated molecular weights of 0.5 X 10(5) to 1.0 X 10(5) and 3 X 10(6). Peak synthesis of the (+) strand viral DNA occurred 50 to 70 min after infection. The initial species detected had a molecular weight of 1.5 X 10(5) to 4.0 X 10(5) which shifted as a function of time to 3 X 10(6). Both (+) strand species were initially detected in the cytoplasm followed by a rapid (10-min interval) appearance of the faster-sedimenting species in the nucleus. The virus-specific (-) and (+) strand DNA species are presumably unintegrated intermediates in provirus formation.
发现劳舍尔白血病病毒（RLV）和莫洛尼肉瘤病毒的RLV假型在小鼠胚胎成纤维细胞中的复制转化功能在感染后30至90分钟对阿糖胞苷（ara-C）的抑制最为敏感。在此时间间隔内通过核酸杂交检测到细胞内RLV DNA合成的起始。用阿糖胞苷处理感染细胞可消除RLV DNA合成。与感染的RLV基因组互补的DNA即（-）链的合成高峰出现在感染后40至60分钟。在此间隔期间观察到两种DNA，估计分子量分别为0.5×10⁵至1.0×10⁵和3×10⁶。（+）链病毒DNA的合成高峰出现在感染后50至70分钟。最初检测到的物种分子量为1.5×10⁵至4.0×10⁵，其随时间变化至3×10⁶。两种（+）链物种最初在细胞质中被检测到，随后较快沉降的物种在细胞核中迅速（10分钟间隔）出现。病毒特异性的（-）和（+）链DNA物种可能是前病毒形成过程中未整合的中间体。
