Katoch V M, Desikan K V
Lepr India. 1983 Apr;55(2):292-8.
The medium 'V' has been evaluated for the in vitro growth of M. leprae. M. leprae were obtained from five untreated cases of multibacillary type of leprosy. Medium was prepared and inoculated by the method described by Veeraraghavan. Killed (Autoclaved) M. leprae from the same biopsies were kept as controls. Growth was monitored in a double blind manner at 60 hours, 120 hours of incubation at 8-10 degrees C of both primary and subcultures. Counting was done by both standard Shepard and McRae method as well as by Veeraraghavan's method. There was no evidence of any multiplication in any of the cases at 60 and 120 hours of incubation.
已对培养基“V”进行了体外麻风分枝杆菌生长评估。从5例未经治疗的多菌型麻风病患者身上获取了麻风分枝杆菌。按照维拉腊格哈万描述的方法制备并接种培养基。取自相同活检样本的经灭活（高压灭菌）的麻风分枝杆菌用作对照。在8-10摄氏度下对原代培养物和传代培养物分别培养60小时和120小时后，以双盲方式监测生长情况。采用标准的谢泼德和麦克雷方法以及维拉腊格哈万方法进行计数。在培养60小时和120小时时，未发现任何病例有增殖迹象。
