Nakamura M, Matsuo Y, Mori T, Okada S, Nakayama T, Saito H, Ito T, Koseki Y, Ogawa T, Ishihara S, Kohsaka K, Nakamura K, Ishidate M
Int J Lepr Other Mycobact Dis. 1982 Dec;50(4):480-7.
Confirmation experiments on colony formation of M. leprae on the M-Y 14b agar medium developed by Murohashi and Yoshida were carried out for two years independently by individual members of an organized research group, according to the method described by Murohashi and Yoshida. The results obtained can be summarized as follows: a) No colony production by M. leprae on M-Y 14b agar medium was seen. b) No increase in the number of cells of M. leprae on M-Y 14b agar medium during cultivation was seen. c) Light and electron microscopic observation indicated that there was an increase in the number of non-solid bacterial cells and ghost cells with time of cultivation. d) It was found by mouse foot pad inoculation that four of six samples of M. leprae used as inocula were definitely viable. e) By means of mouse foot pad inoculation, it was shown that viability of M. leprae inoculated onto M-Y 14b agar medium was lost within approximately seven weeks of cultivation. From these results, can be definitely concluded that there is no evidence indicating that multiplication of M. leprae took place on M-Y 14b agar medium.
由室桥和吉田开发的M - Y 14b琼脂培养基上麻风杆菌菌落形成的验证实验，由一个有组织的研究小组的成员根据室桥和吉田描述的方法独立进行了两年。得到的结果总结如下：a）在M - Y 14b琼脂培养基上未观察到麻风杆菌产生菌落。b）在培养过程中，未观察到M - Y 14b琼脂培养基上麻风杆菌细胞数量增加。c）光学和电子显微镜观察表明，随着培养时间的延长，非固态细菌细胞和空壳细胞的数量增加。d）通过小鼠足垫接种发现，用作接种物的六个麻风杆菌样本中有四个肯定是有活力的。e）通过小鼠足垫接种表明，接种到M - Y 14b琼脂培养基上的麻风杆菌在培养约七周内失去活力。从这些结果可以明确得出结论，没有证据表明麻风杆菌在M - Y 14b琼脂培养基上发生增殖。
