Jacq A, Kohiyama M, Lother H, Messer W
Mol Gen Genet. 1983;191(3):460-5. doi: 10.1007/BF00425763.
The DNA binding protein B' preparation, isolated from the membrane of E. coli, recognizes two sites, one of which is located in the minimum oriC (35-270 bp) and the other between base pairs 417 and 488. Recognition is only possible when restriction fragments containing these sites are in single-stranded state. At the first site the strand reading 3'OH-5'P in the direction of the E. coli genetic map is recognized, at the second site the 5'P-3'OH strand.
从大肠杆菌膜中分离得到的DNA结合蛋白B'制剂可识别两个位点，其中一个位于最小oriC（35 - 270 bp）中，另一个位于碱基对417和488之间。只有当含有这些位点的限制性片段处于单链状态时才可能发生识别。在第一个位点，识别的是沿大肠杆菌遗传图谱方向读取为3'OH - 5'P的链，在第二个位点，识别的是5'P - 3'OH链。
