DnaA蛋白调控的转录：对大肠杆菌微型染色体体外复制的影响
dnaA protein-regulated transcription: effects on the in vitro replication of Escherichia coli minichromosomes.
作者信息
Lother H, Kölling R, Kücherer C, Schauzu M
出版信息
EMBO J. 1985 Feb;4(2):555-60. doi: 10.1002/j.1460-2075.1985.tb03664.x.
Abstract
Both initiation of replication and initiation of transcription are influenced by dnaA protein, when minichromosomes are assayed in vitro for dnaA protein complementation. This dnaA protein effect is seen only if minichromosomes are used containing the 16-kd promoter, from which transcription is directed into the minimal origin. Determination of the 16-kd promoter activity both in vivo and in vitro showed that this strong promoter is specifically repressed by dnaA protein. The 16-kd promoter is thus an integral regulatory region of oriC.
摘要
当在体外检测微型染色体的dnaA蛋白互补时，复制起始和转录起始均受dnaA蛋白影响。只有当使用含有16-kd启动子的微型染色体时，才能观察到这种dnaA蛋白效应，转录从该启动子导向最小的起始点。体内和体外对16-kd启动子活性的测定表明，这种强启动子受到dnaA蛋白的特异性抑制。因此，16-kd启动子是oriC的一个完整调控区域。
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