Kim J H, Kim S H, Hahn E W, Song C W
Science. 1978 Apr 14;200(4338):206-7. doi: 10.1126/science.635582.
To investigate the mechanisms by which heat affects cancer cells, we used 5-thio-D-glucose, an inhibitor of glycolysis in HeLa S-3 cells, under aerobic and hypoxic conditions at temperatures ranging from 37 degrees to 43 degrees C. Drug alone or heat alone killed a minimum number of cells under aerobic or hypoxic conditions. Exposure to drug and hyperthermia selectively increased the number of cells killed under hypoxic conditions at temperatures as low as 40.5 degrees C but had little effect on cells incubated under aerobic conditions. These results suggest that the glycolytic pathways is a primary site of hyperthermic damage leading to cell death.
为了研究热影响癌细胞的机制，我们在37摄氏度至43摄氏度的温度范围内，在有氧和缺氧条件下，使用5-硫代-D-葡萄糖（一种HeLa S-3细胞糖酵解的抑制剂）。单独使用药物或单独加热在有氧或缺氧条件下杀死的细胞数量最少。在低至40.5摄氏度的温度下，暴露于药物和热疗会选择性地增加在缺氧条件下杀死的细胞数量，但对在有氧条件下培养的细胞几乎没有影响。这些结果表明，糖酵解途径是导致细胞死亡的热损伤的主要部位。
