Kaden J, Groth J, Oesterwitz H, Schröder K
Z Urol Nephrol. 1983 Jun;76(6):413-9.
Male BD IX rats were intraperitoneally immunized with 6.35 X 10(6) nylon adherent spleen lymphocytes from male Sprague-Dawley rats (SD). The inbred strains used are different from each other at their major histocompatibility complex. Sensitized animals were exsanguinated 2, 4, 8 and 12 days after the primary injection. After the secondary injection on day 14 the animals were exsanguinated on day 21. The BD IX recipients of (BD IX X SD) F1 renal allograft were intravenously injected with 1 ml of the antisera immediately after completion of the transplant. Significant prolongation of survival time was already obtained with the 8-day-serum pool. The absorption of antiserum with packed SD red blood cells did not affect its enhancing activity. In vitro testing of antisera by 51Cr-release assay showed the highest titer of complement depending cytotoxicity 8 days after immunization. Whilst the cytotoxic activity against nonadherent spleen cells was completely lost after twice absorption with SD erythrocytes, the cytotoxic activity against adherent spleen cells was only slightly reduced. Thus it is likely that enhancing activity of alloantiserum is attributed to anti-Ia or anti-B-cell antibodies. These antibodies were detectable 4 to 8 days after immunization with low dose nylon adherent spleen cells.
将6.35×10⁶来自雄性斯普拉格 - 道利大鼠（SD）的尼龙黏附脾淋巴细胞腹腔内注射给雄性BD IX大鼠进行免疫。所用的近交系在其主要组织相容性复合体上彼此不同。致敏动物在初次注射后2、4、8和12天放血。在第14天进行二次注射后，动物在第21天放血。（BD IX×SD）F1肾移植的BD IX受体在移植完成后立即静脉注射1 ml抗血清。8天血清池已使存活时间显著延长。用SD红细胞压积吸收抗血清不影响其增强活性。通过⁵¹Cr释放试验对抗血清进行体外检测显示，免疫后8天补体依赖性细胞毒性的滴度最高。用SD红细胞两次吸收后，对非黏附脾细胞的细胞毒性活性完全丧失，而对黏附脾细胞的细胞毒性活性仅略有降低。因此，同种异体抗血清的增强活性可能归因于抗Ia或抗B细胞抗体。在用低剂量尼龙黏附脾细胞免疫后4至8天可检测到这些抗体。
