A method for concentration of nucleoside triphosphates by coprecipitation with calcium fluoride.
作者信息
Kukko E I, Kallio T M, Heinonen J K
出版信息
Anal Biochem. 1983 Aug;133(1):58-61. doi: 10.1016/0003-2697(83)90221-x.
PMID:6356980
Abstract
A method for concentration of nucleoside triphosphates (NTP) is described. NTPs are quantitatively coprecipitated from the solution with calcium fluoride. The precipitate is separated by filtration through a membrane filter and NTPs are dissolved from the filter by immersing it in 0.5 N H2SO4. With this method also nucleoside diphosphates can be efficiently concentrated, but the method does not work with nucleoside monophosphates or cyclic AMP.
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