Bertram T A, Coignoul F L, Jensen A E
Am J Vet Res. 1983 Oct;44(10):1923-7.
Equine neutrophils were combined with contagious equine metritis organism (CEMO) or Escherichia coli in vitro in the presence of seminal plasma, uterine flushings, or Hank's balanced salt solution (HBSS). Phagocytosis and intracellular killing were estimated by bacterial culture and light and electron microscopy. With lysed neutrophils, the numbers of colony-forming units of CEMO and E coli increased in seminal plasma and uterine flushings. Numbers of CEMO decreased in HBSS. The numbers of CEMO increased more in the presence of seminal plasma than the other media. When neutrophils were in the various media, 29% to 32% of the CEMO were phagocytized by 120 minutes. At all sampling times and in all media types, more E coli than CEMO were observed to be associated with neutrophils. By 210 minutes' incubation in uterine flushings, seminal plasma, and HBSS, 60% to 75% of the intracellular CEMO were killed. Of the intracellular E coli in the various media, 85% to 90% were killed by 210 minutes. Seemingly, CEMO is an extracellular pathogenic bacteria and immunoglobulins in seminal plasma and uterine flushings from horses not previously infected with CEMO do not enhance CEMO phagocytosis or intracellular killing.
在存在精浆、子宫冲洗液或汉克平衡盐溶液（HBSS）的情况下，将马中性粒细胞与马传染性子宫炎病原体（CEMO）或大肠杆菌在体外混合。通过细菌培养以及光学和电子显微镜来评估吞噬作用和细胞内杀伤情况。对于裂解的中性粒细胞，在精浆和子宫冲洗液中，CEMO和大肠杆菌的菌落形成单位数量增加。在HBSS中，CEMO的数量减少。与其他培养基相比，在精浆存在的情况下，CEMO的数量增加得更多。当中性粒细胞处于各种培养基中时，到120分钟时，29%至32%的CEMO被吞噬。在所有采样时间和所有培养基类型中，观察到与中性粒细胞相关的大肠杆菌比CEMO更多。在子宫冲洗液、精浆和HBSS中孵育210分钟后，60%至75%的细胞内CEMO被杀死。在各种培养基中，细胞内大肠杆菌在210分钟时85%至90%被杀死。似乎，CEMO是一种细胞外病原菌，来自未感染CEMO的马的精浆和子宫冲洗液中的免疫球蛋白不会增强CEMO的吞噬作用或细胞内杀伤。
