Bertram T A, Coignoul F L, Jensen A E
Infect Immun. 1982 Sep;37(3):1241-7. doi: 10.1128/iai.37.3.1241-1247.1982.
Equine neutrophils were combined with Haemophilus equigenitalis (contagious equine metritis organism; CEMO) or Escherichia coli in low- and high-antibody-titer serum to evaluate the neutrophils ability to phagocytize and kill these bacteria. More E. coli than CEMO were phagocytized at each time period. After 120 min in low-antibody-titer serum, 56.3% of the E. coli and 34.3% of the CEMO were phagocytized. A total of 45% of CEMO and 74.9% of E. coli were phagocytized by 120 min when neutrophils were in high-antibody-titer serum. More than 75% of the ingested E. coli and 90% of the ingested CEMO were killed within 210 min of incubation. Fewer E. coli than CEMO were killed at any given time period. Ultrastructural examination showed CEMO to be degraded in the neutrophil. Degradation was the most extensive in neutrophils in high-titer serum. It is suggested that CEMO is a pathogenic extracellular bacterium incapable of prolonged intracellular survival and that it is slower to be phagocytized than a nonpathogenic E. coli.
将马中性粒细胞与马生殖支原体（传染性马子宫内膜炎病原体；CEMO）或大肠杆菌在低抗体滴度和高抗体滴度血清中混合，以评估中性粒细胞吞噬和杀死这些细菌的能力。在每个时间段，被吞噬的大肠杆菌都比CEMO多。在低抗体滴度血清中孵育120分钟后，56.3%的大肠杆菌和34.3%的CEMO被吞噬。当中性粒细胞处于高抗体滴度血清中时，到120分钟时，总共45%的CEMO和74.9%的大肠杆菌被吞噬。在孵育210分钟内，超过75%被吞噬的大肠杆菌和90%被吞噬的CEMO被杀死。在任何给定时间段内，被杀死的大肠杆菌都比CEMO少。超微结构检查显示CEMO在中性粒细胞中被降解。在高滴度血清中的中性粒细胞中，降解最为广泛。提示CEMO是一种致病性胞外细菌，无法在细胞内长期存活，且其被吞噬的速度比非致病性大肠杆菌慢。
