Hancock W W, Zola H, Atkins R C
Blood. 1983 Dec;62(6):1271-9.
Eight monoclonal antibodies to cell surface antigens of human monocytes were evaluated as immunologic markers for recognition of macrophages in sections of normal and diseased tissues, using immunoperoxidase and enzyme histochemical techniques. Monoclonal antibodies assessed were PHM2, PHM3, FMC17, FMC32, FMC33, FMC34, OKM1, and 63D3. Sites studied were human bone marrow, blood, lymph node, spleen, thymus, liver, kidney, lung, and peritoneal lavages, rejecting renal allografts containing inflammatory macrophages, and granulomata showing epithelioid and multinucleate giant cell formation. All antibodies bound to at least some tissue macrophages and, except for FMC32 and FMC33 antibodies, which were identically distributed, each antibody had a distinctive tissue distribution. Some antigens were shared by other bone-marrow-derived cells (megakaryocytes and cortical thymocytes), endothelium, epithelium, and dendritic cells. Antigenic differences were also detected between mononuclear phagocytes present at different sites, different stages of differentiation, and likely different states of activation. These studies provide evidence of major antigenic differences between various populations of human mononuclear phagocytes. They therefore indicate the need for careful evaluation of experiments involving the recognition of macrophages in tissue sections and smears based solely on the use of antimonocyte monoclonal antibodies.
利用免疫过氧化物酶和酶组织化学技术，评估了8种针对人单核细胞表面抗原的单克隆抗体，作为在正常和患病组织切片中识别巨噬细胞的免疫标记物。所评估的单克隆抗体为PHM2、PHM3、FMC17、FMC32、FMC33、FMC34、OKM1和63D3。研究的部位包括人骨髓、血液、淋巴结、脾脏、胸腺、肝脏、肾脏、肺以及腹腔灌洗液，排斥含有炎性巨噬细胞的肾移植异体移植物，以及显示上皮样和多核巨细胞形成的肉芽肿。所有抗体均与至少一些组织巨噬细胞结合，除了分布相同的FMC32和FMC33抗体外，每种抗体都有独特的组织分布。一些抗原也存在于其他骨髓来源的细胞（巨核细胞和皮质胸腺细胞）、内皮细胞、上皮细胞和树突状细胞中。在不同部位、不同分化阶段以及可能不同激活状态的单核吞噬细胞之间也检测到了抗原差异。这些研究提供了证据，证明了人类单核吞噬细胞不同群体之间存在主要的抗原差异。因此，它们表明需要仔细评估仅基于使用抗单核细胞单克隆抗体来识别组织切片和涂片巨噬细胞的实验。
