Schenk J O, Miller E, Rice M E, Adams R N
Brain Res. 1983 Oct 24;277(1):1-8. doi: 10.1016/0006-8993(83)90901-0.
Practical utilization of in vivo electrochemistry for brain studies has been impeded by difficulties with specificity and quantitative measurements. Studies in brain slices combined with similar data from intact animals have provided important new information on the nature of the electrochemical studies. Using the enzyme ascorbic acid oxidase (AAO), the primary signal contribution has been calculated to come from ascorbic acid. By inhibiting monoamine oxidase activity with pargyline, the second most important contributor in caudate extracellular fluid is postulated to be 3,4-dihydroxyphenylacetic acid. These species are calculated to constitute over 93% of the observed oxidation current in rat caudate extracellular fluid.
体内电化学在脑研究中的实际应用受到特异性和定量测量方面困难的阻碍。脑切片研究与来自完整动物的类似数据相结合，为电化学研究的本质提供了重要的新信息。使用抗坏血酸氧化酶（AAO），已计算出主要信号贡献来自抗坏血酸。通过用优降宁抑制单胺氧化酶活性，推测尾状核细胞外液中第二重要的贡献物质是3,4-二羟基苯乙酸。据计算，这些物质在大鼠尾状核细胞外液中构成了超过93%的观察到的氧化电流。
