使用运输增菌培养基检测人工授精公牛中的胎儿弯曲杆菌。
Detection of Campylobacter fetus in artificial insemination bulls with a transport enrichment medium.
作者信息
Garcia M M, Ruckerbauer G M, Eaglesome M D, Boisclair W E
出版信息
Can J Comp Med. 1983 Jul;47(3):336-40.
Abstract
One hundred and five bulls from an artificial insemination unit were tested for the presence of Campylobacter fetus subspecies venerealis. The method involved the inoculation of preputial samples into a new transport enrichment medium prior to culture and immunofluorescence tests. Seventeen bulls (16%) were found to be either positive or suspected carriers of C. fetus at one or more sampling times. The average age of these 17 bulls was about two years greater than the average age of all the bulls in the unit. A combined treatment of vaccination and dihydrostreptomycin sulfate injection suppressed or eliminated the organism from carrier bulls. The use of transport enrichment medium has increased our capability and effectiveness to monitor the presence of C. fetus in artificial insemination bulls.
摘要
对一家人工授精单位的105头公牛进行了胎儿弯曲杆菌性病亚种检测。该方法包括在培养和免疫荧光测试之前，将包皮样本接种到一种新的运输增菌培养基中。在一个或多个采样时间发现17头公牛（16%）为胎儿弯曲杆菌阳性或疑似携带者。这17头公牛的平均年龄比该单位所有公牛的平均年龄大约大两岁。疫苗接种和硫酸双氢链霉素注射的联合治疗抑制或消除了携带者公牛体内的病菌。运输增菌培养基的使用提高了我们监测人工授精公牛中胎儿弯曲杆菌存在情况的能力和效率。
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