Lennarz W J
CRC Crit Rev Biochem. 1983;14(4):257-72. doi: 10.3109/10409238309102795.
One of the most striking morphogenetic events during embryonic development is gastrulation, a process that leads to formation of the primitive gut. Using sea urchin embryos, we have studied the synthesis and function of glycoproteins during gastrulation. These studies have revealed that at least three processes are induced prior to gastrulation: de novo synthesis of dolichol; phosphorylation of dolichol by dolichol kinase, which may catalyze the final step in the de novo pathway; and initiation of the synthesis of N-linked glycoproteins. Whether or not activation of the glycosylation process results merely because of the production of dolichyl monophosphate or because, in addition, proteins containing glycosylatable-Asn-X-Ser/Thr-sequences are first translated just prior to gastrulation, is currently being investigated.
胚胎发育过程中最显著的形态发生事件之一是原肠胚形成，这一过程导致原始肠道的形成。我们利用海胆胚胎研究了原肠胚形成过程中糖蛋白的合成与功能。这些研究表明，在原肠胚形成之前至少诱导了三个过程：多萜醇的从头合成；多萜醇激酶对多萜醇的磷酸化，这可能催化从头合成途径的最后一步；以及N-连接糖蛋白合成的起始。糖基化过程的激活仅仅是由于单磷酸多萜醇的产生，还是除此之外，含有可糖基化的天冬酰胺- X - 丝氨酸/苏氨酸序列的蛋白质在原肠胚形成之前首先被翻译，目前正在研究中。
