Moesgaard F, Lykkegaard Nielsen M C, Justesen T
Eur J Clin Microbiol. 1983 Oct;2(5):459-62. doi: 10.1007/BF02013904.
Wound infection, defined as accumulation of pus draining spontaneously or after opening of the wound, developed in 19 out of 22 guinea pigs (86%) after intraincisional contamination with 10(7) Escherichia coli plus 10(8) Bacteroides fragilis before wound closure. Antibiotic prophylaxis with gentamicin plus clindamycin significantly reduced the wound sepsis rate from 86% to 29% (p less than 0,001). Pus for bacteriological studies was available in all cases of wound infection but one, and culture always revealed both Escherichia coli and Bacteroides fragilis. The animal model presented employs widely accepted criteria for wound infection, and avoids culture of tissue homogenates.
伤口感染定义为伤口自行引流或开放后有脓液积聚，在22只豚鼠中有19只（86%）在伤口闭合前经切口内接种10⁷ 大肠杆菌加10⁸ 脆弱拟杆菌后发生了伤口感染。用庆大霉素加克林霉素进行抗生素预防可将伤口脓毒症发生率从86%显著降低至29%（p<0.001）。除1例伤口感染病例外，所有伤口感染病例均有用于细菌学研究的脓液，培养结果总是显示有大肠杆菌和脆弱拟杆菌。所采用的动物模型采用了广泛接受的伤口感染标准，并且避免了组织匀浆的培养。
