细菌反向突变的选择与终点分布
Selection and endpoint distribution of bacterial inversion mutations.
作者信息
Schmid M B, Roth J R
出版信息
Genetics. 1983 Nov;105(3):539-57. doi: 10.1093/genetics/105.3.539.
Abstract
This paper describes the isolation and characterization of spontaneous inversion mutants of Salmonella typhimurium. The mutants are selected by demanding that an unexpressed hisD gene acquire a new promoter. Chromosome rearrangements that juxtapose the hisD gene and a foreign promoter are obtained by this selection. Although a number of inversions are found, the frequency was lower than expected. The breakpoint of these inversions are not distributed randomly either in the his operon or on the chromosome. The his breakpoint lies in the hisG-hisD intercistronic region, a sequence known to occur at several places on the bacterial chromosome. In most of the inversions, the 'non-his' breakpoint lies across the chromosome, so that the inverted region includes the origin or terminus of DNA replication. The significance of these results is discussed.
摘要
本文描述了鼠伤寒沙门氏菌自发倒位突变体的分离和特性鉴定。通过要求未表达的hisD基因获得一个新启动子来筛选这些突变体。通过这种筛选获得了使hisD基因与外源启动子并列的染色体重排。虽然发现了一些倒位，但频率低于预期。这些倒位的断点在his操纵子或染色体上也不是随机分布的。his断点位于hisG-hisD顺反子间区域，该序列已知在细菌染色体的多个位置出现。在大多数倒位中，“非his”断点位于染色体上，使得倒位区域包括DNA复制的起点或终点。讨论了这些结果的意义。
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