需要腺嘌呤的组氨酸突变体：鼠伤寒沙门氏菌中组氨酸G表达降低的突变体筛选
Histidine mutants requiring adenine: selection of mutants with reduced hisG expression in Salmonella typhimurium.
作者信息
Johnston H M, Roth J R
出版信息
Genetics. 1979 May;92(1):1-15. doi: 10.1093/genetics/92.1.1.
Abstract
A method is described for the selection of Salmonella typhimurium mutants with reduced levels of hisG enzyme activity. This method is based on the fact that the hisG enzyme catalyzes the consumption of ATP in the first step of histidine biosynthesis. Normally, this reaction is closely regulated, both by feedback inhibition and by repression of the operon. However, conditions can be set up that result in the uncontrolled use of adenine in histidine biosynthesis. Cells grown under these conditions become phenotypic adenine auxotrophs. Some revertant clones that no longer require adenine contain mutations in hisG, hisE, or the his-control region. The hisG mutations are of all types (nonsense, frameshift, missense, deletion and leady types), and they map throughout the hisG gene.
摘要
本文描述了一种筛选组氨酸G酶活性水平降低的鼠伤寒沙门氏菌突变体的方法。该方法基于这样一个事实，即组氨酸G酶在组氨酸生物合成的第一步催化ATP的消耗。通常，该反应受到反馈抑制和操纵子阻遏的严格调控。然而，可以设置一些条件，导致在组氨酸生物合成中腺嘌呤的无节制使用。在这些条件下生长的细胞成为表型腺嘌呤营养缺陷型。一些不再需要腺嘌呤的回复克隆在组氨酸G、组氨酸E或组氨酸控制区域含有突变。组氨酸G突变具有所有类型（无义、移码、错义、缺失和迟缓类型），并且它们定位在整个组氨酸G基因中。
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Histidine mutants requiring adenine: selection of mutants with reduced hisG expression in Salmonella typhimurium.需要腺嘌呤的组氨酸突变体：鼠伤寒沙门氏菌中组氨酸G表达降低的突变体筛选
Genetics. 1979 May;92(1):1-15. doi: 10.1093/genetics/92.1.1.
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