Westaway E G, Goodman M R
Arch Virol. 1987;94(3-4):215-28. doi: 10.1007/BF01310715.
Indirect immunofluorescence with rabbit antisera was used to probe the intracellular locations of the antigens of envelope, prM (precursor to structural protein M) and the nonstructural glycoproteins NS 1 (formerly described as NV 3 or SCF) specified by the flaviviruses dengue-2 and Kunjin. Perinuclear staining in various types of foci was prominent for all antigens, and the distribution was influenced by whether cells were fixed with acetone or formaldehyde. Staining of Golgi-like masses or inclusions by anti-envelope sera occurred regularly and prominently in cells infected and stained with homologous anti-envelope antibodies; in the cross reactions, such staining was largely absent, especially in dengue-2 infected cells in which it was replaced by many small circular foci scattered throughout the cytoplasm. Anti-NS 1 also stained large perinuclear inclusions and small cytoplasmic foci, but the distribution of these was dissimilar to that observed with anti-envelope sera. Anti-prM appeared to contain a mixture of antibodies of different specificities, evident at different dilutions, possibly because of different cytoplasmic locations of prM and its cleavage products. All antisera produced small discontinuous foci on the plasma membrane of unfixed infected cells; antigens of NS 1 were sometimes prominent on the surface of acetone-fixed cells.
采用兔抗血清间接免疫荧光法检测黄病毒登革2型和库京病毒包膜抗原、prM（结构蛋白M的前体）和非结构糖蛋白NS 1（以前称为NV 3或SCF）在细胞内的定位。所有抗原在各类病灶中均可见明显的核周染色，其分布受细胞固定剂是丙酮还是甲醛的影响。在用同源抗包膜抗体感染并染色的细胞中，抗包膜血清对高尔基体样团块或包涵体的染色经常且明显；在交叉反应中，这种染色基本不存在，尤其是在登革2型感染的细胞中，取而代之的是散布于整个细胞质中的许多小圆形病灶。抗NS 1也可染出大的核周包涵体和小的细胞质病灶，但它们的分布与抗包膜血清所观察到的不同。抗prM似乎包含不同特异性的抗体混合物，在不同稀释度下可见，这可能是由于prM及其裂解产物在细胞质中的位置不同所致。所有抗血清在未固定的感染细胞的质膜上产生小的间断病灶；NS 1抗原有时在丙酮固定细胞的表面很明显。
