麻疹病毒诱导星形细胞瘤细胞系（U-251）中胶质纤维酸性蛋白细胞骨架的破坏。
Measles virus-induced disruption of the glial-fibrillary-acidic protein cytoskeleton in an astrocytoma cell line (U-251).
作者信息
Duprex W P, McQuaid S, Rima B K
机构信息
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT9 7BL, Northern Ireland, United Kingdom.
出版信息
J Virol. 2000 Apr;74(8):3874-80. doi: 10.1128/jvi.74.8.3874-3880.2000.
Abstract
A recombinant measles virus which expresses enhanced green fluorescent protein (MVeGFP) has been used to infect two astrocytoma cell lines (GCCM and U-251) to study the effect of virus infection on the cytoskeleton. Indirect immunocytochemistry was used to demonstrate the cellular localization of the cytoskeletal components. Enhanced green fluorescent protein autofluorescence was used to identify measles virus-infected cells. No alteration of the actin, tubulin, or vimentin components of the cytoskeleton was observed in either cell type, whereas a disruption of the glial-fibrillary-acidic protein filament (GFAP) network was noted in MVeGFP-infected U-251 cells. The relative amounts of GFAP present in infected and uninfected U-251 cells were quantified by image analysis of data sets obtained by confocal microscopy by using vimentin, another intermediate filament on which MVeGFP has no effect, as a control.
摘要
一种表达增强型绿色荧光蛋白的重组麻疹病毒（MVeGFP）已被用于感染两种星形细胞瘤细胞系（GCCM和U-251），以研究病毒感染对细胞骨架的影响。采用间接免疫细胞化学法来证明细胞骨架成分的细胞定位。利用增强型绿色荧光蛋白自身荧光来识别感染麻疹病毒的细胞。在这两种细胞类型中均未观察到细胞骨架的肌动蛋白、微管蛋白或波形蛋白成分发生改变，而在感染MVeGFP的U-251细胞中，观察到胶质纤维酸性蛋白丝（GFAP）网络遭到破坏。通过共聚焦显微镜获得数据集，以波形蛋白（MVeGFP对其无影响的另一种中间丝）作为对照，通过图像分析对感染和未感染的U-251细胞中GFAP的相对含量进行了定量。
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本文引用的文献
1
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J Virol. 1999 Nov;73(11):9568-75. doi: 10.1128/JVI.73.11.9568-9575.1999.
2
Role of cellular actin in the gene expression and morphogenesis of human respiratory syncytial virus.
Virology. 1998 Dec 5;252(1):137-48. doi: 10.1006/viro.1998.9471.
3
Expression of the enhanced green fluorescent protein by herpes simplex virus type 1 (HSV-1) as an in vitro or in vivo marker for virus entry and replication.单纯疱疹病毒1型（HSV-1）表达增强型绿色荧光蛋白作为病毒进入和复制的体外或体内标志物。
J Virol Methods. 1998 Nov;75(2):151-60. doi: 10.1016/s0166-0934(98)00107-4.
4
Receptor (CD46)- and replication-mediated interleukin-6 induction by measles virus in human astrocytoma cells.麻疹病毒在人星形细胞瘤细胞中通过受体（CD46）和复制介导的白细胞介素-6诱导
J Neurovirol. 1998 Oct;4(5):521-30. doi: 10.3109/13550289809113496.
5
Construction of a mouse hepatitis virus recombinant expressing a foreign gene.表达外源基因的小鼠肝炎病毒重组体的构建。
Adv Exp Med Biol. 1998;440:291-5. doi: 10.1007/978-1-4615-5331-1_38.
6
Infectious simian varicella virus expressing the green fluorescent protein.表达绿色荧光蛋白的传染性猴水痘病毒。
J Neurovirol. 1998 Aug;4(4):438-44. doi: 10.3109/13550289809114543.
7
Measles virus antigen in macrophage/microglial cells and astrocytes of subacute sclerosing panencephalitis.
APMIS. 1998 May;106(5):553-61. doi: 10.1111/j.1699-0463.1998.tb01384.x.
8
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Virus Res. 1998 Jan;53(1):13-25. doi: 10.1016/s0168-1702(97)00121-4.
9
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J Virol. 1998 Apr;72(4):2655-62. doi: 10.1128/JVI.72.4.2655-2662.1998.
10
Measles virus infections of the central nervous system.麻疹病毒引起的中枢神经系统感染。
Intervirology. 1997;40(2-3):176-84. doi: 10.1159/000150544.
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