Brown P J, Molloy C M, Johnson P M
J Reprod Immunol. 1983 Nov;5(6):351-61. doi: 10.1016/0165-0378(83)90244-9.
Human trophoblast membrane antigens recognised by monoclonal antibodies (H310, H315, H316 and H317) have been identified using combinations of radioimmunoprecipitation, SDS-PAGE, electroblotting, chromatographic and ELISA-type techniques. H317 is known to identify heat-stable placental-type alkaline phosphatase and accordingly was shown to react with a protein of subunit Mr of 68 000. H310 and H316 both recognise an antigen with a subunit Mr of 34 000 under reducing conditions. In non-reducing conditions, the H310/316 antigen gave oligomers of a component of Mr 62 000. It is unknown whether this 62 000 dalton component is a dimer of the 34 000 dalton protein with either itself or a second protein chain of presumed Mr around 28 000. H315 recognises an antigen with subunit Mr of 36 000; in non-reducing conditions this component readily associates to oligomeric structures. The epitope recognised by H315 may be sensitive to SDS. The two proteins recognised by H310/316 and H315 have been termed the p34 and p36 trophoblast membrane proteins, respectively.
利用放射免疫沉淀、SDS - PAGE、电印迹、色谱和ELISA类技术组合，已鉴定出可被单克隆抗体（H310、H315、H316和H317）识别的人滋养层膜抗原。已知H317可识别热稳定的胎盘型碱性磷酸酶，因此显示其与亚基Mr为68000的一种蛋白质发生反应。在还原条件下，H310和H316均识别亚基Mr为34000的一种抗原。在非还原条件下，H310/316抗原产生Mr为62000的一种成分的寡聚体。尚不清楚这种62000道尔顿的成分是34000道尔顿蛋白质自身的二聚体，还是与假定Mr约为28000的第二条蛋白质链形成的二聚体。H315识别亚基Mr为36000的一种抗原；在非还原条件下，该成分易于缔合形成寡聚结构。H315识别的表位可能对SDS敏感。被H310/316和H315识别的两种蛋白质分别被称为p34和p36滋养层膜蛋白。
