Price M R, Campbell D G, Baldwin R W
Scand J Immunol. 1983 Nov;18(5):411-20. doi: 10.1111/j.1365-3083.1983.tb00873.x.
An anti-human osteogenic sarcoma monoclonal antibody (mouse IgG2b) termed 791T/36 was found to exert complement-dependent cytotoxicity against phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear (PBMN) cells. This reaction was examined by flow cytofluorimetry using indirect membrane immunofluorescence to detect cell-bound antibody and by measurement of the binding of fluorescein-isothiocyanate-conjugated 791T/36 antibody to cells. The antibody reacted strongly with peripheral blood blast cells induced by PHA, and there was negligible reactivity with resting lymphocytes. Maximum binding was observed after 3 days' culture with PHA, coinciding with maximum DNA synthesis, and this represented of the order of 2 X 10(5) antibody molecules bound per cell. After cell surface radioiodination of PHA-stimulated PBMN cells, detergent lysis and immunoprecipitation of antigen with 791T/36 antibody and Sepharose-protein A, the apparent molecular weight of this antigen was determined to be 72,000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. This is identical to that of the 791T/36-defined antigen expressed on various osteogenic sarcoma cell lines [3, 17], and by this criterion the antigen is distinguishable from other cell surface markers of activated human lymphocytes.
一种名为791T/36的抗人骨肉瘤单克隆抗体（小鼠IgG2b）被发现对植物血凝素（PHA）刺激的外周血单个核（PBMN）细胞具有补体依赖性细胞毒性。通过使用间接膜免疫荧光的流式细胞荧光测定法检测细胞结合抗体，并通过测量异硫氰酸荧光素偶联的791T/36抗体与细胞的结合来检查这种反应。该抗体与PHA诱导的外周血母细胞强烈反应，而与静息淋巴细胞的反应可忽略不计。在与PHA培养3天后观察到最大结合，这与最大DNA合成同时发生，并且这代表每个细胞结合约2×10⁵个抗体分子。在用PHA刺激的PBMN细胞进行细胞表面放射性碘化、去污剂裂解并用791T/36抗体和琼脂糖蛋白A进行抗原免疫沉淀后，通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳确定该抗原的表观分子量为72,000。这与在各种骨肉瘤细胞系上表达的791T/36定义的抗原相同[3,17]，并且根据这一标准，该抗原可与活化的人淋巴细胞的其他细胞表面标志物区分开来。
