Tesliar G E, Shavlovskiĭ G M
Tsitol Genet. 1983 Sep-Oct;17(5):54-6.
A conjugation analysis of riboflavin-dependent mutants of Escherichia coli K-12 has been made by means of various F'-factors. It is shown that the GTP cyclohydrolase II gene is localized in the chromosome map site between 27 and 30 min; the riboflavin synthase gene--in the site between 56 and 59 min; and the gene in which mutation causes accumulation of 2,6-dioxy-5-amino-4-ribitylamino-pyrimidine--in the site limited by 61-65 min. So it may be concluded that riboflavin biosynthesis genes in the E. coli chromosome are not clustered.
利用各种F'因子对大肠杆菌K-12的核黄素依赖性突变体进行了接合分析。结果表明，GTP环水解酶II基因位于染色体图谱上27至30分钟之间的位点；核黄素合酶基因位于56至59分钟之间的位点；而突变导致2,6-二氧-5-氨基-4-核糖基氨基嘧啶积累的基因位于61至65分钟限定的位点。因此可以得出结论，大肠杆菌染色体中的核黄素生物合成基因不是成簇的。
