核黄素的生物合成：大肠杆菌中编码GTP环化水解酶II的基因的克隆、测序、定位及表达
Biosynthesis of riboflavin: cloning, sequencing, mapping, and expression of the gene coding for GTP cyclohydrolase II in Escherichia coli.
作者信息
Richter G, Ritz H, Katzenmeier G, Volk R, Kohnle A, Lottspeich F, Allendorf D, Bacher A
机构信息
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Garching, Germany.
出版信息
J Bacteriol. 1993 Jul;175(13):4045-51. doi: 10.1128/jb.175.13.4045-4051.1993.
Abstract
GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated an open reading frame of 588 bp coding for a 21.8-kDa peptide of 196 amino acids. The gene was mapped to a position at 28.2 min on the E. coli chromosome and is identical with ribA. GTP cyclohydrolase II was overexpressed in a recombinant strain carrying a plasmid with the cloned gene. The enzyme was purified to homogeneity from the recombinant strain. The N-terminal sequence determined by Edman degradation was identical to the predicted sequence. The sequence is homologous to the 3' part of the central open reading frame in the riboflavin operon of Bacillus subtilis.
摘要
GTP环化水解酶II催化核黄素生物合成中的首个关键步骤。通过标记拯救技术已克隆出大肠杆菌中编码该酶的基因。测序表明有一个588 bp的开放阅读框，编码一个由196个氨基酸组成的21.8 kDa肽段。该基因定位于大肠杆菌染色体上28.2分钟处的位置，与ribA相同。GTP环化水解酶II在携带含有克隆基因质粒的重组菌株中过表达。该酶从重组菌株中纯化至同质。通过埃德曼降解法测定的N端序列与预测序列相同。该序列与枯草芽孢杆菌核黄素操纵子中央开放阅读框的3'部分同源。
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本文引用的文献
1
Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein.考马斯亮蓝G染料结合法测定蛋白质时，使对不同蛋白质的反应变化最小化。
Anal Biochem. 1981 Sep 1;116(1):53-64. doi: 10.1016/0003-2697(81)90321-3.
2
3
[3 linkage groups of the genes of riboflavin biosynthesis in Escherichia coli].[大肠杆菌中核黄素生物合成基因的3个连锁群]
Genetika. 1983 Sep;19(9):1419-25.
4
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
Gene. 1982 Oct;19(3):259-68. doi: 10.1016/0378-1119(82)90015-4.
5
A novel cloning vector for the direct selection of recombinant DNA in E. coli.一种用于在大肠杆菌中直接筛选重组DNA的新型克隆载体。
Gene. 1982 Sep;19(2):231-4. doi: 10.1016/0378-1119(82)90011-7.
6
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.一种制备大量φX174 RF 1超螺旋DNA的简单方法。
Biochim Biophys Acta. 1973 Apr 11;299(4):516-20. doi: 10.1016/0005-2787(73)90223-2.
9
[Study of the 210-degree region of the Bacillus subtilis chromosome using recombinant plasmids].[利用重组质粒对枯草芽孢杆菌染色体210度区域的研究]
Mol Gen Mikrobiol Virusol. 1987 Feb(2):20-4.
10
The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.完整大肠杆菌染色体的物理图谱：一种用于大型基因组文库快速分析和分类的新策略的应用
Cell. 1987 Jul 31;50(3):495-508. doi: 10.1016/0092-8674(87)90503-4.
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