Brown G V, Stace J D, Anders R F
Am J Trop Med Hyg. 1983 Nov;32(6):1221-8. doi: 10.4269/ajtmh.1983.32.1221.
In the search for antibodies correlating with host-protective immunity to Plasmodium falciparum in man, sera from individuals in Papua New Guinea were analyzed at the time of infection and in the convalescent period following infection. Titers of antibody were determined by enzyme linked immunoassay (ELISA), and the specificities of antibodies was examined by gel electrophoresis of immunoprecipitates. In the majority of cases, convalescence was associated with an increase in antibody titer and one-dimensional gel analysis of immunoprecipitated biosynthetically labeled parasite antigens demonstrated the variability in specificity of the antibody response in the two types of serum samples from different individuals. A protein of Mr 96,000 which has previously been identified as a candidate host-protective antigen was not clearly seen in immunoprecipitates generated with acute serum, even in samples with high titers of antibody assessed by ELISA. Antibodies to a protein Mr 96,000 were present in some, but not all convalescent sera. Two-dimensional gel analysis was more sensitive in detecting a boost in antibody response to minor antigens (e.g., an acidic protein Mr approximately equal to 230,000). This approach has not led to identification of antibody specificities to major antigens which are invariably boosted by infection and drug cure, but has identified antibody specificities in acute sera which are inadequate in quantity or quality to inhibit parasite growth.
在寻找与人类对恶性疟原虫的宿主保护性免疫相关的抗体过程中，对巴布亚新几内亚个体在感染时及感染后的恢复期血清进行了分析。通过酶联免疫吸附测定（ELISA）确定抗体滴度，并通过免疫沉淀物的凝胶电泳检查抗体的特异性。在大多数情况下，恢复期与抗体滴度升高相关，对免疫沉淀的生物合成标记寄生虫抗原进行的一维凝胶分析表明，来自不同个体的两种类型血清样本中抗体反应的特异性存在差异。一种分子量为96,000的蛋白质，先前已被鉴定为候选宿主保护性抗原，即使在ELISA评估抗体滴度较高的样本中，在用急性血清产生的免疫沉淀物中也未清晰可见。分子量为96,000的蛋白质抗体在一些但并非所有恢复期血清中存在。二维凝胶分析在检测对次要抗原（例如，一种分子量约为230,000的酸性蛋白质）的抗体反应增强方面更为敏感。这种方法尚未导致鉴定出对主要抗原的抗体特异性，这些主要抗原在感染和药物治愈后总是会增强，但已鉴定出急性血清中抗体特异性，其数量或质量不足以抑制寄生虫生长。
