Hirsh D C, Martin L D
Appl Environ Microbiol. 1983 Nov;46(5):1243-5. doi: 10.1128/aem.46.5.1243-1245.1983.
A method is described whereby the presence of less than five salmonellae was detected per milliliter of milk within 24 h of sample collection. Salmonellae were removed from milk by means of electropositive large-pore filters. Eluates from the filters were analyzed for the presence of Salmonella spp. by Felix-O1 bacteriophage and high-pressure liquid chromatographic techniques. The method gave only a positive response when salmonellae were present in the milk. Of the serotypes and strains of Salmonella spp. tested, Salmonella dublin (10 strains), Salmonella typhimurium (5 strains), Salmonella anatum, Salmonella krefeld, and Salmonella saint-paul gave positive responses. One strain of Salmonella agona (three strains tested) and three strains of Salmonella enteritidis (seven strains tested) were not detectable by the method described herein.
本文描述了一种方法，通过该方法可在采集样品后的24小时内检测出每毫升牛奶中沙门氏菌含量低于5个。利用带正电的大孔过滤器从牛奶中去除沙门氏菌。通过费利克斯 - O1噬菌体和高压液相色谱技术分析过滤器洗脱液中沙门氏菌属的存在情况。该方法仅在牛奶中存在沙门氏菌时给出阳性反应。在所测试的沙门氏菌属血清型和菌株中，都柏林沙门氏菌（10株）、鼠伤寒沙门氏菌（5株）、鸭沙门氏菌、克雷费尔德沙门氏菌和圣保罗沙门氏菌给出了阳性反应。该方法无法检测出1株阿哥纳沙门氏菌（共测试3株）和3株肠炎沙门氏菌（共测试7株）。
