用O-1噬菌体鉴定沙门氏菌。
Identification of Salmonella with the O-1 bacteriophage.
作者信息
Welkos S, Schreiber M, Baer H
出版信息
Appl Microbiol. 1974 Oct;28(4):618-22. doi: 10.1128/am.28.4.618-622.1974.
Abstract
The O-1 bacteriophage test of Cherry et al. (1954) for the presumptive identification of salmonellae in the diagnostic laboratory was investigated. A phage lysate with a titer of 10(12) plaque-forming units per ml was found to be optimal. This preparation lysed 98.2% of Salmonella strains tested, while maintaining its high specificity for salmonellae. Gram-negative organisms other than salmonellae were resistant to the O-1 phage; however, 5.9% of Escherichia coli strains tested were susceptible. The O-1 phage test is a simple, rapid, inexpensive, sensitive, and specific procedure for the identification of salmonellae in the diagnostic laboratory. A presumptive identification is obtained 1 day earlier than with conventional biochemical tests.
摘要
对Cherry等人（1954年）用于诊断实验室中沙门氏菌初步鉴定的O-1噬菌体试验进行了研究。发现每毫升效价为10(12) 噬菌斑形成单位的噬菌体裂解物是最佳的。该制剂裂解了98.2% 的测试沙门氏菌菌株，同时保持了对沙门氏菌的高特异性。除沙门氏菌外的革兰氏阴性菌对O-1噬菌体具有抗性；然而，5.9% 的测试大肠杆菌菌株易感。O-1噬菌体试验是诊断实验室中鉴定沙门氏菌的一种简单、快速、廉价、灵敏且特异的方法。与传统生化试验相比，可提前1天获得初步鉴定结果。
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