Wells J G, Morris G K, Brachman P S
Appl Microbiol. 1971 Feb;21(2):235-9. doi: 10.1128/am.21.2.235-239.1971.
The use of a cotton gauze swab and subsequent culture of the swab was found to be a more sensitive method for isolating Salmonella from liquid milk than the revised procedure of North. The swab method was found to be as sensitive as the North procedure for recovering Salmonella when incubated at 37 C but more sensitive when incubated at 43 C. Incubation of the swab cultures at the elevated temperature of 43 C gave good results when Salmonella was present at levels as low as one per liter. Swabs exposed to milk contaminated with 100 Salmonella per liter remained positive even when subsequently washed for 2 hr in noncontaminated milk. Bismuth sulfite agar and Brilliant Green sulfadiazine agar were equally effective for isolating Salmonella from broth cultures; use of both media resulted in maximal isolations.
研究发现，与诺斯修订后的方法相比，使用棉拭子并随后对拭子进行培养是从液态奶中分离沙门氏菌更灵敏的方法。当在37℃培养时，拭子法在回收沙门氏菌方面与诺斯方法一样灵敏，但在43℃培养时更灵敏。当沙门氏菌含量低至每升一个时，将拭子培养物在43℃的高温下培养可得到良好结果。暴露于每升含有100个沙门氏菌的污染牛奶中的拭子，即使随后在未污染的牛奶中洗涤2小时仍保持阳性。亚硫酸铋琼脂和煌绿磺胺嘧啶琼脂在从肉汤培养物中分离沙门氏菌方面同样有效；同时使用这两种培养基可实现最大程度的分离。
