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利用费利克斯-O1噬菌体和高效液相色谱法快速检测沙门氏菌属

Rapid detection of Salmonella spp. by using Felix-O1 bacteriophage and high-performance liquid chromatography.

作者信息

Hirsh D C, Martin L D

出版信息

Appl Environ Microbiol. 1983 Jan;45(1):260-4. doi: 10.1128/aem.45.1.260-264.1983.

Abstract

A method is described whereby the presence of Salmonella spp. can be detected within 8 to 24 h of sample collection. The method depends upon the interaction of Salmonella spp. with the Salmonella-specific Felix-O1 bacteriophage. This interaction results in an increase in concentration of the bacteriophage which is detected by high-performance liquid chromatographic techniques.

摘要

本文描述了一种方法,通过该方法可在采集样品后的8至24小时内检测出沙门氏菌属的存在。该方法依赖于沙门氏菌属与沙门氏菌特异性费利克斯-O1噬菌体的相互作用。这种相互作用导致噬菌体浓度增加,可通过高效液相色谱技术检测到。

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本文引用的文献

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Rapid radiometric method for detection of Salmonella in foods.食品中沙门氏菌检测的快速辐射测量法。
Appl Environ Microbiol. 1980 Aug;40(2):223-30. doi: 10.1128/aem.40.2.223-230.1980.
6
Enzyme immunoassay for detection of Salmonellae in foods.用于检测食品中沙门氏菌的酶免疫测定法。
Appl Environ Microbiol. 1982 Apr;43(4):877-93. doi: 10.1128/aem.43.4.877-893.1982.
9
Sensitivity of various salmonella strains to felix 0-1 phage.
Acta Pathol Microbiol Scand. 1967;70(3):446-54. doi: 10.1111/j.1699-0463.1967.tb01312.x.
10
Identification of Salmonella with the O-1 bacteriophage.用O-1噬菌体鉴定沙门氏菌。
Appl Microbiol. 1974 Oct;28(4):618-22. doi: 10.1128/am.28.4.618-622.1974.

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