Hyperacute rejection of xenografts. II. Isolation of individual components of the graft effluent serum.

Separation of glycoprotein components from Con A-Sepharose in acid solution gave an electrophoretic picture of transplant serum containing a "double band" which was not found on any electrophoresis of fractions eluted from Con A-Sepharose at pH 7.4. This band corresponded with mobility to proteins of molecular weight higher than heavy IgG chains. We assume that at pH 3 the observed picture of the new protein may be a glycoprotein antigen dissociated from the immunological complex. Immunophoresis of the transplant serum fraction confirmed our assumption that at pH 3 there appeared a new component absent at pH 7.4. Our attempts at separating this component on Sephadex G-150 were unsuccessful.