Wachal T, Olszewski W L, Ruka M
Arch Immunol Ther Exp (Warsz). 1983;31(2):237-41.
Separation of glycoprotein components from Con A-Sepharose in acid solution gave an electrophoretic picture of transplant serum containing a "double band" which was not found on any electrophoresis of fractions eluted from Con A-Sepharose at pH 7.4. This band corresponded with mobility to proteins of molecular weight higher than heavy IgG chains. We assume that at pH 3 the observed picture of the new protein may be a glycoprotein antigen dissociated from the immunological complex. Immunophoresis of the transplant serum fraction confirmed our assumption that at pH 3 there appeared a new component absent at pH 7.4. Our attempts at separating this component on Sephadex G-150 were unsuccessful.
在酸性溶液中从刀豆球蛋白A-琼脂糖凝胶上分离糖蛋白成分，得到移植血清的电泳图谱，其中含有一条“双带”，而在pH 7.4条件下从刀豆球蛋白A-琼脂糖凝胶上洗脱的各组分的任何电泳图谱中均未发现此带。该条带的迁移率与分子量高于重链IgG的蛋白质相对应。我们推测，在pH 3时观察到的新蛋白质图谱可能是从免疫复合物中解离出来的一种糖蛋白抗原。移植血清组分的免疫电泳证实了我们的推测，即在pH 3时出现了一种在pH 7.4时不存在的新组分。我们尝试在葡聚糖凝胶G-150上分离该组分，但未成功。
