Schmidt M A, Jann B, Jann K
Eur J Biochem. 1983 Dec 1;137(1-2):163-71. doi: 10.1111/j.1432-1033.1983.tb07810.x.
The O-specific polysaccharide moiety (04 polysaccharide) of the 04 antigen (lipopolysaccharide) from Escherichia coli 04:K12:H- was isolated in pure form by degradation of the lipopolysaccharide and chromatography on Sephadex G-50. The primary structure of the 04 polysaccharide was elucidated by composition, Smith degradation, nuclear magnetic resonance spectroscopy, methylation and oligosaccharide analysis. Oligosaccharides were obtained by deaminating fragmentation of partially and completely de-N-acetylated polysaccharides. The polysaccharide consists of repeating units of the pentasaccharide (formula; see text) which are joined in the polymer through alpha-1, 4-linkages. The 04 polysaccharide has a mean molecular mass of 13800 Da and consists of the core oligosaccharide and about 14 pentasaccharide repeating units.
通过对脂多糖进行降解并在葡聚糖G - 50上进行色谱分离，从大肠杆菌O4:K12:H - 的O4抗原（脂多糖）中以纯形式分离出O - 特异性多糖部分（O4多糖）。通过组成分析、史密斯降解、核磁共振光谱、甲基化和寡糖分析阐明了O4多糖的一级结构。寡糖是通过对部分和完全脱N - 乙酰化多糖进行脱氨基断裂获得的。该多糖由五糖（化学式；见正文）的重复单元组成，这些重复单元通过α - 1,4 - 连接在聚合物中相连。O4多糖的平均分子量为13800 Da，由核心寡糖和约14个五糖重复单元组成。
