大肠杆菌O18ac抗原：O-特异性多糖部分的结构
Escherichia coli O18ac antigen: structure of the O-specific polysaccharide moiety.
作者信息
Gupta D S, Jann B, Jann K
出版信息
Infect Immun. 1984 Jul;45(1):203-9. doi: 10.1128/iai.45.1.203-209.1984.
Abstract
The O-specific polysaccharide moiety (O18ac polysaccharide) of the O18ac antigen (lipopolysaccharide) from Escherichia coli 2980 (O18ac:K5:Fim+:H-) was isolated in pure form by degradation of the lipopolysaccharide and chromatography on Sephadex G-50. The primary structure of the O18ac polysaccharide was elucidated by composition, fragmentation procedures, methylation analysis, and nuclear magnetic resonance spectroscopy. The polysaccharide consists of repeating units of the pentasaccharide: (formula; see text) which are joined in the polymer by alpha-1,2 linkages.
摘要
通过对脂多糖进行降解并在葡聚糖G - 50上进行色谱分离，从大肠杆菌2980（O18ac:K5:Fim+:H-）的O18ac抗原（脂多糖）中以纯形式分离出O特异性多糖部分（O18ac多糖）。通过组成分析、片段化程序、甲基化分析和核磁共振光谱法阐明了O18ac多糖的一级结构。该多糖由五糖的重复单元组成：（化学式；见正文），这些重复单元在聚合物中通过α-1,2键连接。
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