大肠杆菌O18ac抗原：O-特异性多糖部分的结构
Escherichia coli O18ac antigen: structure of the O-specific polysaccharide moiety.
作者信息
Gupta D S, Jann B, Jann K
出版信息
Infect Immun. 1984 Jul;45(1):203-9. doi: 10.1128/iai.45.1.203-209.1984.
Abstract
The O-specific polysaccharide moiety (O18ac polysaccharide) of the O18ac antigen (lipopolysaccharide) from Escherichia coli 2980 (O18ac:K5:Fim+:H-) was isolated in pure form by degradation of the lipopolysaccharide and chromatography on Sephadex G-50. The primary structure of the O18ac polysaccharide was elucidated by composition, fragmentation procedures, methylation analysis, and nuclear magnetic resonance spectroscopy. The polysaccharide consists of repeating units of the pentasaccharide: (formula; see text) which are joined in the polymer by alpha-1,2 linkages.
摘要
通过对脂多糖进行降解并在葡聚糖G - 50上进行色谱分离，从大肠杆菌2980（O18ac:K5:Fim+:H-）的O18ac抗原（脂多糖）中以纯形式分离出O特异性多糖部分（O18ac多糖）。通过组成分析、片段化程序、甲基化分析和核磁共振光谱法阐明了O18ac多糖的一级结构。该多糖由五糖的重复单元组成：（化学式；见正文），这些重复单元在聚合物中通过α-1,2键连接。
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本文引用的文献
1
Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin.
J Biol Chem. 1959 Dec;234:3263-8.
2
The application of 500-MHz H-NMR spectroscopy for the structure elucidation of N-acetyllactosamine type asparagine-bound carbohydrate chains of glycoproteins.
FEBS Lett. 1980 May 19;114(1):11-6. doi: 10.1016/0014-5793(80)80850-7.
3
Structures of fifteen oligosaccharides isolated from new-born meconium.
Eur J Biochem. 1981 Jul;117(2):291-300. doi: 10.1111/j.1432-1033.1981.tb06336.x.
4
The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin.
Eur J Biochem. 1981 May 15;116(2):359-64. doi: 10.1111/j.1432-1033.1981.tb05343.x.
5
Structural studies of the O-specific side chain of the lipopolysaccharide from Escherichia coli O:7.
Carbohydr Res. 1984 Mar 15;126(2):249-59. doi: 10.1016/0008-6215(84)85382-3.
6
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Eur J Biochem. 1983 Dec 1;137(1-2):163-71. doi: 10.1111/j.1432-1033.1983.tb07810.x.
7
Six widespread bacterial clones among Escherichia coli K1 isolates.
Infect Immun. 1983 Jan;39(1):315-35. doi: 10.1128/iai.39.1.315-335.1983.
8
Induction of bacteremia in newborn rats by Escherichia coli K1 is correlated with only certain O (lipopolysaccharide) antigen types.
Infect Immun. 1983 Feb;39(2):599-608. doi: 10.1128/iai.39.2.599-608.1983.
9
Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli.
Infect Immun. 1984 Jan;43(1):368-79. doi: 10.1128/iai.43.1.368-379.1984.
10
Characterization of pili associated with Escherichia coli O18ac.
Infect Immun. 1980 Aug;29(2):685-91. doi: 10.1128/iai.29.2.685-691.1980.
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