Dorne A M, Eneas-Filho J, Heizmann P, Mache R
Mol Gen Genet. 1984;193(1):129-34. doi: 10.1007/BF00327425.
A comparison of ribosomal proteins from Escherichia coli and from chloroplasts of Spinach was made using two separate methods: electrophoretic migration and immunochemical cross-reaction between blotted E. coli ribosomal proteins and chloroplast ribosomal subunits antisera. It is shown that L2 from E. coli (E-L2) and L4 from chloroplasts (CS-L4) comigrated and that E-L2 immunologically cross-reacted with the isolated CS-L4 antibody. Co-migration was observed for three additional couples of 50S ribosomal proteins. It is also shown that at least one 30S E. coli ribosomal protein immuno-cross reacted with a 30S chloroplast antiserum and that three couples of 30S ribosomal proteins comigrated.
电泳迁移以及印迹的大肠杆菌核糖体蛋白与叶绿体核糖体亚基抗血清之间的免疫化学交叉反应。结果表明,大肠杆菌的L2(E-L2)与叶绿体的L4(CS-L4)迁移率相同,且E-L2与分离出的CS-L4抗体发生免疫交叉反应。另外还观察到三对50S核糖体蛋白的迁移率相同。研究还表明,至少有一种30S大肠杆菌核糖体蛋白与30S叶绿体抗血清发生免疫交叉反应,并且有三对30S核糖体蛋白迁移率相同。