菠菜叶绿体中rpl23、rpl2和rps19基因的表达

Thomas F, Massenet O, Dorne A M, Briat J F, Mache R

Laboratoire de Biologie Moléculaire Végétale, CNRS UA 1178, Université de Grenoble, St Martin d'Hères, France.

Nucleic Acids Res. 1988 Mar 25;16(6):2461-72. doi: 10.1093/nar/16.6.2461.

The expression of the spinach rpl23, rpl2 and rps19 chloroplast genes has been studied. The rpl23 gene identified in tobacco and Marchantia, is split into two overlapping reading frames in spinach. S1 mapping has shown that initiation sites could occur upstream of each reading frames. A large transcription unit is also present covering the rpl2 and rps19 genes. The rps19 and rpl2 gene products are identified by NH2-terminal amino acid sequences. They correspond to spinach chloroplast ribosomal proteins CS-S23 and CS-L4, respectively. No product of the rpl23 gene was detected in the chloroplast 50S ribosomal subunit. This strongly suggest that a corresponding gene has been transfered into the nucleus.

对菠菜叶绿体基因rpl23、rpl2和rps19的表达进行了研究。在烟草和地钱中鉴定出的rpl23基因，在菠菜中被分成两个重叠的阅读框。S1图谱显示起始位点可能出现在每个阅读框的上游。还存在一个覆盖rpl2和rps19基因的大转录单元。通过NH2末端氨基酸序列鉴定了rps19和rpl2基因产物。它们分别对应于菠菜叶绿体核糖体蛋白CS-S23和CS-L4。在叶绿体50S核糖体亚基中未检测到rpl23基因的产物。这强烈表明相应的基因已转移到细胞核中。