Hozoc M, Nastac E, Suru M, Stoian M, Bercovici S, Cajal N
Virologie. 1983 Oct-Dec;34(4):273-82.
Inoculation of ultraviolet (UV)-irradiated adenovirus type 4 (Ad4) led to in vitro transformation of human diploid cells (HDC). Two transformed cell lines could be established: cell line H 1418, from HDC inoculated with the 10(-3) dilution of Ad4 UV-irradiated for 20 min at a distance of 20 cm, co-cultivated with uninfected HDC, and cell line H 1557, from HDC inoculated with the 10(-2) dilution of Ad4 irradiated at the same distance for 12 min. Both transformed cell lines were resistant to superinfection with homologous virus. Virus-specific antigen could be made evident by the indirect immunofluorescence technique in the nuclei of both H 1418 and H 1557 cells.
接种经紫外线（UV）照射的4型腺病毒（Ad4）可导致人二倍体细胞（HDC）发生体外转化。可建立两个转化细胞系：细胞系H 1418，由在20 cm距离处经紫外线照射20分钟的Ad4的10⁻³稀释液接种的HDC与未感染的HDC共同培养得到；细胞系H 1557，由在相同距离处经紫外线照射12分钟的Ad4的10⁻²稀释液接种的HDC得到。两个转化细胞系均对同源病毒的超感染具有抗性。通过间接免疫荧光技术可在H 1418和H 1557细胞的细胞核中检测到病毒特异性抗原。
