Salminen A, Komulainen J, Ahomäki E, Kainulainen H, Takala T, Vihko V
Acta Physiol Scand. 1983 Nov;119(3):261-5. doi: 10.1111/j.1748-1716.1983.tb07336.x.
This study aimed at comparing the effects of running and swimming training protocols and the termination of training on the activities of two proteases with alkaline pH-optima (alkaline protease and myofibrillar protease) in the tibialis anterior, soleus, and gastrocnemius muscles of male rats. The training on treadmill decreased the activities of alkaline and myofibrillar proteases by approx. 10-20% in the muscles studied. The activities of both proteases were unchanged in swimming-trained rats. Two weeks after the termination of running training the activity of alkaline protease was increased in gastrocnemius muscle but not in the other muscles. Swimming training increased the activity of citrate synthase in all muscles studied but training by running only in the soleus muscle. The running protocol increased the activity of beta-glucuronidase in the tibialis anterior muscle and decreased the activity in the gastrocnemius muscle. The swimming program did not affect beta-glucuronidase activities. These results show diverse effects of running and swimming training on alkaline proteolytic activities as well as on mitochondrial and lysosomal marker enzymes.
本研究旨在比较跑步和游泳训练方案以及训练终止对雄性大鼠胫骨前肌、比目鱼肌和腓肠肌中两种最适pH呈碱性的蛋白酶（碱性蛋白酶和肌原纤维蛋白酶）活性的影响。跑步机训练使所研究肌肉中的碱性蛋白酶和肌原纤维蛋白酶活性降低了约10 - 20%。游泳训练大鼠的两种蛋白酶活性均未改变。跑步训练终止两周后，腓肠肌中碱性蛋白酶的活性增加，但其他肌肉中未增加。游泳训练使所有所研究肌肉中的柠檬酸合酶活性增加，但跑步训练仅使比目鱼肌中的柠檬酸合酶活性增加。跑步方案增加了胫骨前肌中β - 葡萄糖醛酸酶的活性，降低了腓肠肌中该酶的活性。游泳方案未影响β - 葡萄糖醛酸酶的活性。这些结果表明，跑步和游泳训练对碱性蛋白水解活性以及线粒体和溶酶体标记酶具有不同的影响。
