Salminen A, Kihlström M, Kainulainen H, Takala T, Vihko V
Eur J Appl Physiol Occup Physiol. 1984;52(3):287-90. doi: 10.1007/BF01015211.
Alkaline and myofibrillar protease activities of rectus femoris, soleus, and tibialis anterior muscles and the pooled sample of gastrocnemius and plantaris muscles were analyzed in male NMRI-mice during a running-training program of 3, 10, or 20 daily 1-h sessions. The activity of citrate synthase increased during the endurance training, reflecting the increased oxidative capacity of skeletal muscles. The activities of alkaline and myofibrillar proteases continually decreased in the course of the training program in all muscles studied. Instead, the activity of beta-glucuronidase (a marker of lysosomal hydrolases) increased in all muscles. The highest activities were observed at the beginning of the training program. Present results, together with our earlier observations, show that the type of training, running as opposed to swimming, modulates the training responses in alkaline protease activities. Further, diverse adaptations in the activities of alkaline proteases and a lysosomal hydrolase suggest difference in the function of different proteolytic systems.
在雄性NMRI小鼠进行为期3、10或20天、每天1小时的跑步训练计划期间，分析了股直肌、比目鱼肌、胫骨前肌以及腓肠肌和跖肌混合样本的碱性蛋白酶和肌原纤维蛋白酶活性。在耐力训练期间，柠檬酸合酶的活性增加，反映出骨骼肌氧化能力的增强。在所有研究的肌肉中，碱性蛋白酶和肌原纤维蛋白酶的活性在训练过程中持续下降。相反，β-葡萄糖醛酸酶（溶酶体水解酶的标志物）的活性在所有肌肉中均增加。在训练计划开始时观察到最高活性。目前的结果与我们早期的观察结果表明，与游泳相反的跑步这种训练类型，会调节碱性蛋白酶活性的训练反应。此外，碱性蛋白酶和溶酶体水解酶活性的不同适应性表明不同蛋白水解系统的功能存在差异。
