Phillips K R, Pik J, Lawford H G, Lavers B, Kligerman A, Lawford G R
Can J Microbiol. 1983 Oct;29(10):1331-8. doi: 10.1139/m83-207.
The biosynthesis of a thermogelable, extracellular homo-beta-(1 leads to 3)-glucan called "curdlan," has been studied in batch and continuous cultures of Alcaligenes faecalis var. myxogenes. Curdlan production is associated with the poststationary phase of a nitrogen-depleted, aerobic batch culture. Exopolymer is not detected in single-stage, carbon-limited continuous cultures but curdlan can be isolated from the effluent of a nitrogen-limited chemostat operating at a dilution rate (D) of less than 0.1 h-1. A spontaneous variant of strain ATCC 21680 was isolated and found to be compatible with long-term, nitrogen-limited chemostat culture. The specific rate of curdlan production is approximately four times higher in poststationary batch cultures than in single-stage continuous fermentations. The product yield (Yp/S) associated with batch processing (nongrowing cultures) is approximately 0.5 g curdlan/g glucose, with CO2 being the only detectable by-product.
在粪产碱菌黏液变种的分批培养和连续培养中，对一种名为“凝胶多糖”的可热凝胶化细胞外同型β-(1→3)-葡聚糖的生物合成进行了研究。凝胶多糖的产生与氮耗尽的需氧分批培养的稳定后期相关。在单级、碳限制的连续培养中未检测到胞外聚合物，但凝胶多糖可从稀释率（D）小于0.1 h⁻¹运行的氮限制恒化器流出物中分离出来。分离出了菌株ATCC 21680的一个自发变体，发现其与长期的氮限制恒化器培养相容。在稳定后期的分批培养中，凝胶多糖的比生产速率比单级连续发酵中大约高四倍。与分批处理（非生长培养物）相关的产物产率（Yp/S）约为0.5 g凝胶多糖/g葡萄糖，二氧化碳是唯一可检测到的副产物。
