Hendry J H, Moore J V, Potten C S
Cell Tissue Kinet. 1984 Jan;17(1):41-7. doi: 10.1111/j.1365-2184.1984.tb00566.x.
The technique of thymidine (TdR) suicide has been used with the intestinal microcolony assay to demonstrate that in the middle of the light cycle, nearly all intestinal clonogenic cells, in the B6D2F1 mice used in these experiments, were not in S phase. Doses of tritiated thymidine [3H]TdR up to 1 mCi/mouse did not kill a significant fraction of those clonogenic cells which survived a test dose of 12 Gy gamma-rays. This finding supports some data in the literature, but conflicts with others. However, the suicide technique was found in the studies reported here to be very efficient in sterilizing clonogenic cells in the middle of the dark cycle, and also in a regenerating epithelium at day 3 after a dose of 9 Gy. This implies that the technique can discriminate well between populations of clonogenic cells which differ in their content of cells in S phase. The lack of a suicide effect in the middle of the light cycle indicates that the majority of proliferative epithelial cells are not clonogenic.
已将胸苷（TdR）自杀技术与肠道微集落测定法结合使用，以证明在光周期的中间时段，在这些实验中所用的B6D2F1小鼠中，几乎所有肠道克隆形成细胞都不在S期。高达1毫居里/小鼠的氚标记胸苷[³H]TdR剂量并未杀死在经受12 Gyγ射线测试剂量后存活下来的那些克隆形成细胞中的很大一部分。这一发现支持了文献中的一些数据，但与其他数据相矛盾。然而，在此处报告的研究中发现，自杀技术在黑暗周期中间时段对克隆形成细胞进行绝育非常有效，并且在9 Gy剂量照射后第3天的再生上皮中也是如此。这意味着该技术能够很好地区分处于S期的细胞含量不同的克隆形成细胞群体。在光周期中间时段缺乏自杀效应表明，大多数增殖性上皮细胞不是克隆形成细胞。
