Branlant G, Flesch G, Branlant C
Gene. 1983 Nov;25(1):1-7. doi: 10.1016/0378-1119(83)90161-0.
Recombinant plasmids derived from pBR322, which carry gap genes coding for the D-glyceraldehyde-3-phosphate dehydrogenases (GAPDH) of Bacillus stearothermophilus and Escherichia coli, have been isolated. The selection was carried out by complementation of an E. coli gapam mutant. Two plasmids containing B. stearothermophilus and E. coli DNA inserts of 4.3 kb and 1.4 kb, respectively, were characterized. Transformation of the E. coli mutant with either of the recombinant plasmids lead to a very high expression of the GAPDH activity. GAPDH produced by the strain containing the B. stearothermophilus gap gene was characterized by immunological cross-reactivity with antiserum raised against pure B. stearothermophilus GAPDH.
已分离出源自pBR322的重组质粒，这些质粒携带编码嗜热脂肪芽孢杆菌和大肠杆菌的D-甘油醛-3-磷酸脱氢酶（GAPDH）的缺口基因。通过大肠杆菌gapam突变体的互补作用进行筛选。对分别含有4.3 kb和1.4 kb嗜热脂肪芽孢杆菌和大肠杆菌DNA插入片段的两种质粒进行了表征。用任一重组质粒转化大肠杆菌突变体都会导致GAPDH活性的高表达。含有嗜热脂肪芽孢杆菌gap基因的菌株产生的GAPDH的特征是与针对纯嗜热脂肪芽孢杆菌GAPDH产生的抗血清具有免疫交叉反应性。
