缺失gapA和gapB基因的大肠杆菌菌株的特性分析
Characterization of Escherichia coli strains with gapA and gapB genes deleted.
作者信息
Seta F D, Boschi-Muller S, Vignais M L, Branlant G
机构信息
Laboratoire d'Enzymologie et de Génie Génétique, Université Henri Poincaré Nancy I, URA CNRS 457, Vandoeuvre-les-Nancy, France.
出版信息
J Bacteriol. 1997 Aug;179(16):5218-21. doi: 10.1128/jb.179.16.5218-5221.1997.
Abstract
We obtained a series of Escherichia coli strains in which gapA, gapB, or both had been deleted. Delta gapA strains do not revert on glucose, while delta gapB strains grow on glycerol or glucose. We showed that gapB-encoded protein is expressed but at a very low level. Together, these results confirm the essential role for gapA in glycolysis and show that gapB is dispensable for both glycolysis and the pyridoxal biosynthesis pathway.
摘要
我们获得了一系列缺失gapA、gapB或两者均缺失的大肠杆菌菌株。缺失gapA的菌株在葡萄糖上不能回复生长，而缺失gapB的菌株能在甘油或葡萄糖上生长。我们发现gapB编码的蛋白有表达，但水平很低。这些结果共同证实了gapA在糖酵解中的重要作用，并表明gapB对于糖酵解和吡哆醛生物合成途径都是可有可无的。
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