Aoki N, Gerber M A, Thung S N, Chen M L, Christman J K, Price P M, Flordellis C S, Acs G
Hepatology. 1984 Jan-Feb;4(1):84-9. doi: 10.1002/hep.1840040115.
Cultured 3T3 mouse fibroblasts transfected with cloned hepatitis B virus genome and DNA coding for methotrexate-resistant dihydrofolate reductase, produce and secrete significant amounts of hepatitis B surface antigen (HBsAg). Ultrastructural morphometry revealed that fibroblasts transfected with hepatitis B virus DNA contained significantly more lysosomes than did fibroblasts transfected with the gene coding for methotrexate resistance or normal fibroblasts. Although abundant HBsAg was found in the cytoplasm of transfected fibroblasts by immunologic methods, HBsAg particles were not detected by electron microscopy. Immunoelectron microscopy localized HBsAg to the nuclear envelope, rough endoplasmic reticulum, and endoplasmic cisternae. These findings suggest that the transfected cells produce mainly nonparticulate HBsAg or that they have a defect in intracisternal packaging of HBsAg into particles.
用克隆的乙型肝炎病毒基因组和编码对氨甲蝶呤耐药的二氢叶酸还原酶的DNA转染的培养的3T3小鼠成纤维细胞，产生并分泌大量的乙型肝炎表面抗原（HBsAg）。超微结构形态计量学显示，用乙型肝炎病毒DNA转染的成纤维细胞比用编码氨甲蝶呤耐药的基因转染的成纤维细胞或正常成纤维细胞含有明显更多的溶酶体。尽管通过免疫学方法在转染的成纤维细胞的细胞质中发现了大量的HBsAg，但通过电子显微镜未检测到HBsAg颗粒。免疫电子显微镜将HBsAg定位到核膜、粗面内质网和内质池。这些发现表明，转染的细胞主要产生非颗粒性HBsAg，或者它们在将HBsAg包装到颗粒的内质网池中有缺陷。
