大肠杆菌中不耐热肠毒素的细胞定位
Cellular location of heat-labile enterotoxin in Escherichia coli.
作者信息
Hirst T R, Randall L L, Hardy S J
出版信息
J Bacteriol. 1984 Feb;157(2):637-42. doi: 10.1128/jb.157.2.637-642.1984.
Abstract
We demonstrated that both the A and B subunits of heat-labile enterotoxin from Escherichia coli are located in the periplasm. The toxin was shown to form aggregates in Tris-EDTA buffers which are routinely used for isolating membranes. The aggregates pellet upon centrifugation, and this may explain why several previous investigators have concluded that enterotoxin is associated with membranes.
摘要
我们证明了大肠杆菌不耐热肠毒素的A亚基和B亚基都位于周质中。研究表明，该毒素在常用于分离膜的Tris-EDTA缓冲液中会形成聚集体。聚集体在离心时会沉淀，这或许可以解释为什么之前的一些研究人员得出肠毒素与膜相关的结论。
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