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Microtubule-associated protein MAP1 promotes microtubule assembly in vitro.微管相关蛋白MAP1在体外促进微管组装。
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Purification of high-Mr microtubule proteins MAP1 and MAP2.高分子量微管蛋白MAP1和MAP2的纯化
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Molecular analysis of cytoplasmic microtubules in situ: identification of both widespread and specific proteins.原位细胞质微管的分子分析：广泛存在及特异性蛋白质的鉴定
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A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies.一种用于选择和克隆杂交瘤以生产单克隆抗体的简单、一步法技术。
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A dot-immunobinding assay for monoclonal and other antibodies.一种用于单克隆抗体及其他抗体的斑点免疫结合测定法。
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Initial phase of dendrite growth: evidence for the involvement of high molecular weight microtubule-associated proteins (HMWP) before the appearance of tubulin.树突生长的初始阶段：在微管蛋白出现之前高分子量微管相关蛋白（HMWP）参与其中的证据。
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High molecular weight microtubule-associated proteins are preferentially associated with dendritic microtubules in brain.高分子量微管相关蛋白优先与大脑中的树突状微管相关联。
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Microtubule-associated proteins: a monoclonal antibody to MAP2 binds to differentiated neurons.微管相关蛋白：一种针对MAP2的单克隆抗体可与分化的神经元结合。
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Brain postsynaptic densities: the relationship to glial and neuronal filaments.脑突触后致密物：与神经胶质丝和神经元丝的关系。
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使用单克隆抗体对大鼠小脑微管相关蛋白1进行免疫细胞化学定位。

Immunocytochemical localization of microtubule-associated protein 1 in rat cerebellum using monoclonal antibodies.

作者信息

Huber G, Matus A

出版信息

J Cell Biol. 1984 Feb;98(2):777-81. doi: 10.1083/jcb.98.2.777.

DOI:10.1083/jcb.98.2.777
PMID:6363428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2113089/
Abstract

Immunohistochemical staining with monoclonal antibodies showed that microtubule-associated protein 1 (MAP1) has a restricted cellular distribution in the rat cerebellum. Anti-MAP1 staining was found only in neurons, where it was much stronger in dendrites than in axons. There were striking variations in the apparent concentration of MAP1 in different classes of neurons. Purkinje cells were the most strongly labeled, while granule cell neurons gave a faint, threshold-level reaction with the antibody. The reaction of Golgi neurons was intermediate between these two extremes. Equivalent results were obtained using two different methods of tissue preparation. Thus MAP1 appears to be a neuron-specific protein that is highly concentrated in dendrites and occurs at markedly different levels in different types of neurons. These observations provide further indications of heterogeneity among brain microtubules.

摘要

用单克隆抗体进行的免疫组织化学染色显示，微管相关蛋白1（MAP1）在大鼠小脑中具有局限的细胞分布。抗MAP1染色仅在神经元中发现，在树突中的染色比轴突中要强得多。不同类型神经元中MAP1的表观浓度存在显著差异。浦肯野细胞的染色最强，而颗粒细胞神经元与抗体的反应微弱，仅为阈值水平。高尔基神经元的反应介于这两个极端之间。使用两种不同的组织制备方法获得了相同的结果。因此，MAP1似乎是一种神经元特异性蛋白，高度集中在树突中，并且在不同类型的神经元中含量明显不同。这些观察结果进一步表明脑微管之间存在异质性。