Huber G, Matus A
J Cell Biol. 1984 Feb;98(2):777-81. doi: 10.1083/jcb.98.2.777.
Immunohistochemical staining with monoclonal antibodies showed that microtubule-associated protein 1 (MAP1) has a restricted cellular distribution in the rat cerebellum. Anti-MAP1 staining was found only in neurons, where it was much stronger in dendrites than in axons. There were striking variations in the apparent concentration of MAP1 in different classes of neurons. Purkinje cells were the most strongly labeled, while granule cell neurons gave a faint, threshold-level reaction with the antibody. The reaction of Golgi neurons was intermediate between these two extremes. Equivalent results were obtained using two different methods of tissue preparation. Thus MAP1 appears to be a neuron-specific protein that is highly concentrated in dendrites and occurs at markedly different levels in different types of neurons. These observations provide further indications of heterogeneity among brain microtubules.
用单克隆抗体进行的免疫组织化学染色显示,微管相关蛋白1(MAP1)在大鼠小脑中具有局限的细胞分布。抗MAP1染色仅在神经元中发现,在树突中的染色比轴突中要强得多。不同类型神经元中MAP1的表观浓度存在显著差异。浦肯野细胞的染色最强,而颗粒细胞神经元与抗体的反应微弱,仅为阈值水平。高尔基神经元的反应介于这两个极端之间。使用两种不同的组织制备方法获得了相同的结果。因此,MAP1似乎是一种神经元特异性蛋白,高度集中在树突中,并且在不同类型的神经元中含量明显不同。这些观察结果进一步表明脑微管之间存在异质性。