An ultrastructural immunocytochemical study of nerve-specific protein in rat cerebellum.

The ultrastructural localization of the neuron-specific enolase (14-3-2 protein) has been investigated in the cerebellum of the adult rat using the indirect antibody immunohistochemical method. The protein was found exclusively in neurons: perikaryal cytoplasm, axons and dendrites were labelled while nuclei were not. Reaction product was found to be attached to intracytoplasmic membranes, the surface membranes of mitochondria and microtubules in addition to its dispersion as a flocculent material throughout the cytoplasm. All classes of cerebellar neurons were found to be labelled though large variations in the level of labelling between different types of neuron were noted. Purkinje cells appeared to have a much lower cytoplasmic concentration of this protein than other neurons.