Eldred W D, Karten H J
J Comp Neurol. 1983 Dec 20;221(4):371-81. doi: 10.1002/cne.902210402.
Immunocytochemical methods were used for selective labeling and characterization of amacrine cells of the turtle (Pseudemys scripta elegans) retina which contained neuropeptide-like immunoreactivity (leu-enkephalin, glucagon, and neurotensin). Processes of amacrine cells arborized in specific strata of the inner plexiform layer (IPL). Different strata were defined in relation to the boundaries of the IPL. Zero represented the strata nearest the inner nuclear layer and 100 represented the strata nearest the ganglion cells. Antisera directed against leu-enkephalin labeled approximately 7,300 amacrine cells in a single turtle retina which were concentrated in the region of the visual streak and decreased in density toward the periphery. In retinal regions outside the visual streak the labeled neurons were similar in size and shape with dendritic arbors which lacked a particular orientation. In contrast, in the visual streak, there were particular neurons which were labeled with enkephalin antiserum which had elongated dendritic arbors that ran parallel to the streak. Both types of amacrine cells with enkephalinergic immunoreactivity sent their dendrites into the 0-20 region and the 65-100 region of the IPL. Antisera directed against glucagon labeled approximately 2,500 amacrine cells in a single turtle retina. These cells were concentrated in areas near the visual streak. Amacrine cells labeled with glucagon antiserum had dendritic arbors which were asymmetrically skewed toward one end of the cell and ramified in the 0-20 strata with sparse projections at the 40 and 80 strata of the IPL. Antisera directed against neurotensin labeled 12,800 amacrine cells in a single turtle retina. These cells were concentrated in the region of the visual streak. Two distinct amacrine cell types were labeled selectively. One type had a large, vertically oriented cell body (10 X 14 micron) which gave rise to a single 2-micron-thick process that branched and ramified within the 45-70 strata. The other amacrine cell type with neurotensin-like immunoreactivity had a smaller cell body (8 micron) that sent numerous thin dendrites into the same 45-70 strata. The present results indicated that various neuropeptides were present in amacrine cells of the turtle retina and that a specific neuropeptide could be found in more than one anatomical type of amacrine cell. Each anatomical type of amacrine cell had a unique dendritic arborization which ramified within particular strata within the IPL.
免疫细胞化学方法用于对乌龟（滑龟指名亚种）视网膜中含有神经肽样免疫反应性物质（亮脑啡肽、胰高血糖素和神经降压素）的无长突细胞进行选择性标记和特征描述。无长突细胞的突起在内网状层（IPL）的特定层中分支。根据IPL的边界定义了不同的层。零表示最靠近内核层的层，100表示最靠近神经节细胞的层。针对亮脑啡肽的抗血清在单个乌龟视网膜中标记了约7300个无长突细胞，这些细胞集中在视带区域，并且从视带向周边密度逐渐降低。在视带以外的视网膜区域，标记的神经元在大小和形状上相似，其树突分支没有特定的方向。相反，在视带中，有特定的神经元被脑啡肽抗血清标记，其树突分支细长且与视带平行。两种具有脑啡肽能免疫反应性的无长突细胞都将其树突发送到IPL的0 - 20区域和65 - 100区域。针对胰高血糖素的抗血清在单个乌龟视网膜中标记了约2500个无长突细胞。这些细胞集中在视带附近的区域。用胰高血糖素抗血清标记的无长突细胞具有不对称地向细胞一端倾斜的树突分支，并在IPL的0 - 20层中分支，在40层和80层中有稀疏的投射。针对神经降压素的抗血清在单个乌龟视网膜中标记了12800个无长突细胞。这些细胞集中在视带区域。选择性地标记了两种不同类型的无长突细胞。一种类型具有大的、垂直定向的细胞体（10×14微米），产生一个单一的2微米厚的突起，该突起在45 - 70层内分支。另一种具有神经降压素样免疫反应性的无长突细胞类型具有较小的细胞体（8微米），将许多细树突发送到相同的45 - 70层。目前的结果表明，乌龟视网膜的无长突细胞中存在各种神经肽，并且在不止一种解剖学类型的无长突细胞中可以发现特定的神经肽。每种解剖学类型的无长突细胞都有独特的树突分支，在IPL内的特定层中分支。
