Riviere G R
J Dent Res. 1984 Jan;63(1):41-3. doi: 10.1177/00220345840630010901.
Three strain combinations of in-bred mice representing strong and moderate histocompatibility barriers were used to determine if tooth allotransplants evoked strong or weak immunologic memory as measured by the second-set donor-strain skin graft technique. At issue was whether the anatomic site of engraftment could influence the outcome of these experiments. Recipients received either 5-mm-diameter skin grafts or one or four adult teeth. Experimental transplants were placed in subcutaneous pouches or in ear pouches. Control mice received only orthotopic skin grafts. Second-set orthotopic skin grafts were placed either one mo, six mo, or 11 mo after primary transplants. Skin grafts were observed daily until rejection occurred. We found that skin provoked strong memory in subcutaneous tissues, but that teeth generated very weak memory in subcutaneous tissues. Conversely, teeth placed in ear pouches were able to generate strong immunologic memory. This occurred whether donor and recipient shared H-2 allo-antigens, or whether one tooth or four teeth were grafted, and the difference persisted for at least six mo. We conclude that teeth are not weak antigens, nor is the subcutaneous site privileged regarding immunologic memory. Rather, there seems to be some critical interaction between teeth and the immune system draining subcutaneous tissues that results in a failure to generate strong anamnestic immunity. Perhaps definition of the processes involved will be of advantage in future human applications.
使用代表强和中度组织相容性屏障的近交系小鼠的三种品系组合，通过二次供体品系皮肤移植技术来确定牙齿同种异体移植引发的免疫记忆是强还是弱。问题在于移植的解剖部位是否会影响这些实验的结果。受体接受直径5毫米的皮肤移植或一颗或四颗成年牙齿。实验性移植物置于皮下袋或耳袋中。对照小鼠仅接受原位皮肤移植。二次原位皮肤移植在初次移植后1个月、6个月或11个月进行。每天观察皮肤移植，直至发生排斥反应。我们发现皮肤在皮下组织中引发强烈的记忆，但牙齿在皮下组织中产生非常弱的记忆。相反，置于耳袋中的牙齿能够产生强烈的免疫记忆。无论供体和受体是否共享H-2同种抗原，也无论移植一颗牙齿还是四颗牙齿，这种情况都会发生，并且这种差异至少持续6个月。我们得出结论，牙齿不是弱抗原，皮下部位在免疫记忆方面也没有特殊优势。相反，牙齿与引流皮下组织的免疫系统之间似乎存在某种关键的相互作用，导致无法产生强烈的回忆性免疫。也许对所涉及过程的定义在未来的人类应用中会有帮助。
