使用硝酸纤维素“多盘”同时检测与多种变应原结合的IgE。

Simultaneous detection of IgE binding to several allergens using a nitrocellulose 'polydisc'.

作者信息

Walsh B J, Wrigley C W, Baldo B A

出版信息

J Immunol Methods. 1984 Jan 20;66(1):99-102. doi: 10.1016/0022-1759(84)90252-7.

Abstract

The use of nitrocellulose for allergen disc preparation permits several different allergens to be applied as separate spots to the same disc for simultaneous evaluation with one serum sample (50 microliter). To achieve this, an immunological application method involving anti-human IgE and the peroxidase-anti-peroxidase procedure must be used with a substrate that produces an insoluble product. The suitability of the polydiscs for routine clinical evaluation of several allergens at once was demonstrated by testing the sera of seven allergic bakers with discs containing extracts of flour or pollen from wheat, cereal rye, or rye grass.

摘要

使用硝酸纤维素制备过敏原盘，可将几种不同的过敏原作为单独的斑点应用于同一张盘上，以便用一份血清样本（50微升）同时进行检测。为此，必须使用一种涉及抗人IgE和过氧化物酶-抗过氧化物酶程序的免疫应用方法，并使用能产生不溶性产物的底物。通过用含有小麦、黑麦谷物或黑麦草的面粉或花粉提取物的盘检测七名过敏性面包师的血清，证明了这种多盘对于同时对几种过敏原进行常规临床评估的适用性。