Ageenko A I, Vitorgan Iu E, Chernomordik A E
Vopr Onkol. 1978;24(1):51-5.
Isoenzymes of lactate dehydrogenase were studied by disc-electrophoresis in polyacrylamide gel, and in the clinic--in 1% agar gel. Oncovirus A12 invasion of the culture of rat embryo fibroblasts (REF) was found to result in the increased percentage of the cathode fractions activity (LDG-4 and LD-5) and in the disappearance of LDG-1 yet during the first day of the experiment prior to hypoxia and enhanced proliferation, i. e. it is most likely to be primary. In the homogenates of cancerous tumor and large intestine polyps of man also a reliable increase of the cathode and a decrease or disappearance of the anode fractions accur. A correlation of the experimental and clinical data allowed a suggestion to be made that LDG isoenzymes changes are genetically conditioned and play an important role in the process of oncogenesis, providing conditions for the increased intensity of glycolysis and proliferation.
采用聚丙烯酰胺凝胶圆盘电泳法对乳酸脱氢酶同工酶进行了研究，并在临床上采用1%琼脂糖凝胶法进行研究。发现致癌病毒A12侵袭大鼠胚胎成纤维细胞（REF）培养物，会导致阴极部分活性（LDG-4和LD-5）百分比增加，并且在实验的第一天，即在缺氧和增殖增强之前，LDG-1就消失了，也就是说，这很可能是原发性的。在人类癌性肿瘤和大肠息肉的匀浆中，阴极部分也可靠地增加，阳极部分减少或消失。实验数据与临床数据的相关性使得人们可以提出这样的建议，即LDG同工酶的变化是由遗传决定的，并且在肿瘤发生过程中起重要作用，为糖酵解强度和增殖的增加提供条件。
