检测细菌菌落中糖苷酶的改进方法。

Paoni N F, Arroyo R L

Appl Environ Microbiol. 1984 Jan;47(1):208-9. doi: 10.1128/aem.47.1.208-209.1984.

An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to enable better penetration by chromagenic substrates.

已开发出一种检测琼脂平板表面生长菌落中细菌糖苷酶的测定方法。该技术相对于先前描述的方法的优点包括无需影印接种、显色反应产物的可视化效果更好，以及有一个简单的通透步骤以使显色底物能更好地渗透。