Fuccello A, Audhya T, Talle M A, Goldstein G
Arch Biochem Biophys. 1984 Jan;228(1):292-8. doi: 10.1016/0003-9861(84)90070-5.
A polyclonal rabbit anti-bovine thymopoietin antiserum was used to develop a radioimmunoassay and sandwich enzyme-linked immunosorbent (ELISA) assay for the thymic hormone thymopoietin. Both assays showed slightly less sensitivity for the closely related splenic hormone splenin (SP) than thymopoietin (TP) and markedly less sensitivity for the human as compared with the bovine polypeptides. A number of murine monoclonal antibodies specific for bovine thymopoietin were generated; they were unreactive with bovine splenin and were also unreactive with human thymopoietin and splenin. A sandwich ELISA using these monoclonal anti-TP antibodies together with polyclonal rabbit anti-TP was specific for bovine thymopoietin and measured 300-500 ng/ml thymopoietin in bovine serum. Similar approaches are being pursued to develop an immunoassay for thymopoietin in human serum.
使用多克隆兔抗牛胸腺生成素抗血清开发了一种用于胸腺激素胸腺生成素的放射免疫测定法和夹心酶联免疫吸附（ELISA）测定法。两种测定法对密切相关的脾脏激素脾素（SP）的敏感性略低于胸腺生成素（TP），并且与牛多肽相比，对人多肽的敏感性明显更低。产生了许多对牛胸腺生成素具有特异性的鼠单克隆抗体；它们与牛脾素无反应，与人胸腺生成素和脾素也无反应。使用这些抗TP单克隆抗体与多克隆兔抗TP一起的夹心ELISA对牛胸腺生成素具有特异性，并测定牛血清中胸腺生成素的含量为300 - 500 ng/ml。正在采用类似方法开发人血清中胸腺生成素的免疫测定法。
